Journal: Molecular Cell

Article Title: Immune-regulated IDO1-dependent tryptophan metabolism is source of one-carbon units for pancreatic cancer and stellate cells

doi: 10.1016/j.molcel.2021.03.019

Figure Lengend Snippet:

Article Snippet: Rabbit monoclonal anti-IDO1 [mouse] (D8W5E) , CST , Cat#51851.

Techniques: Recombinant, Staining, Western Blot, Expressing, Plasmid Preparation, Cloning, RNAscope, Positive Control, Software

Journal: Cell

Article Title: Dietary and Microbial Oxazoles Induce Intestinal Inflammation by Modulating Aryl Hydrocarbon Receptor Responses

doi: 10.1016/j.cell.2018.04.037

Figure Lengend Snippet: (A) Recombinant IDO1 was incubated with L-tryptophan in the presence of oxazole (oxazolone, TMO, Frag-oz), control (TMC) compounds, or vehicle and formyl-kynurenine production measured via calorimetric assay. ***p < 0.001 (Student’s t test)

Article Snippet: IDO1 Inhibitor Screening Assay Kit , BPS Biosciences , Cat# 72021.

Techniques: Recombinant, Incubation

Journal: Cell

Article Title: Dietary and Microbial Oxazoles Induce Intestinal Inflammation by Modulating Aryl Hydrocarbon Receptor Responses

doi: 10.1016/j.cell.2018.04.037

Figure Lengend Snippet: IECs promote mucosal barrier protection through microsomal triglyceride protein (MTP)-mediated CD1d-restricted production of IL-10 (blue circles). Upon exposure to oxazole compounds (derived from diet, microbes, or other environmental sources), these CD1d-restricted responses are attenuated through a mechanism involving the production of tryptophan-derived metab-olites (kynurenic acid, xanthurenic acid; orange squares) through the activity of IDO1 that trigger the AhR. Decreased, CD1d-restricted IL-10 production results in unrestrained inflammatory responses (IL-13/IFNγ, red dots) and resulting pathology. ER, endoplasmic reticulum.

Article Snippet: IDO1 Inhibitor Screening Assay Kit , BPS Biosciences , Cat# 72021.

Techniques: Derivative Assay, Activity Assay

Journal: Cell

Article Title: Dietary and Microbial Oxazoles Induce Intestinal Inflammation by Modulating Aryl Hydrocarbon Receptor Responses

doi: 10.1016/j.cell.2018.04.037

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: IDO1 Inhibitor Screening Assay Kit , BPS Biosciences , Cat# 72021.

Techniques: Recombinant, Enzyme-linked Immunosorbent Assay, Screening Assay, Luciferase, SYBR Green Assay, Software, Microscopy, Mass Spectrometry

Journal: bioRxiv

Article Title: Human DUX4 and mouse Dux interact with STAT1 and broadly inhibit interferon-stimulated gene induction

doi: 10.1101/2022.08.09.503314

Figure Lengend Snippet: ( A ) FSHD MB200 myoblasts were differentiated into myotubes, which results in the expression of endogenous DUX4 in a subset of myotubes. Cultures were treated ±IFNγ, and DUX4 and IDO1 were visualized by immunofluorescence. A representative image of DUX4+ and DUX4-myotubes shows IDO1 induction only in the DUX4-myotubes (arrows). ( B, left panel ) RT-qPCR of the indicated genes in MB135 parental or Kitra-SRS that express a CIC DUX4-fusion gene containing the DUX4 CTD. Cells were transfected with control or CIC- and DUX4-targeting siRNAs. Ct values were normalized to the house-keeping gene RPL27 . Data represent the mean ±SD of three biological replicates with three technical replicates each. ( B, right panel ) Western blot showing lysates from MB135 or Kitra-SRS cells treated with control or CIC- and DUX4-targeting siRNAs ±IFNγ and probed with the indicated antibodies. See Source Data for uncropped/raw Western blots. ( C ) RT-qPCR of the indicated genes in MB135 with an inducible CIC (MB135-iCIC) or an inducible CIC-DUX4 fusion gene (MB135-iCIC-DUX4). Cells were untreated, IFNγ-treated, or IFNγ and doxycycline treated. Ct values were normalized to the housekeeping gene RPL27 , then normalized to the IFNγ-only treatment to set the induced level to 100%. Data represent the mean ±SD of three biological replicates with three technical replicates each.

Article Snippet: Antibodies against DDX3X [D19B4] (8192S, RRID:AB_10860416), hnRNPK [R332] (4675S, RRID:AB_10622190), TRIM28 [C42G12] (4124S, RRID:AB_2209886), PPP2R1A [81G5] (2041S), NCL [D4C7O] (14574, RRID:AB_2798519), MYC tag [71D10] (2278, RRID:AB_490778), IDO1 [D5J4E] (86630, RRID:AB_2636818), pSTAT1 Y701 [58D6] (9167, RRID:AB_561284), and Phospho-Rbp1 CTD (Ser5) [D9N5I] (13523, RRID:AB_2798246) were purchased from Cell Signaling Technology.

Techniques: Expressing, Immunofluorescence, Quantitative RT-PCR, Transfection, Control, Western Blot

Journal: Stem Cells International

Article Title: The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

doi: 10.1155/2019/4592701

Figure Lengend Snippet: The expression of TRAIL in AFMSCs was initiated by both IFN- β and IFN- γ , but only IFN- γ initiated the expression of IDO1 in AFMSCs. (a) AFMSCs were primed for 12 hours with IFN- β or IFN- γ (20 ng/ml each). TRAIL and IDO1 mRNA were measured by real-time PCR, and GAPDH was used as an internal control. The expression levels were normalized to the level of GAPDH mRNA (defined as an arbitrary unit). (b) AFMSCs were primed with IFN- β or IFN- γ at specified concentrations for 12 hours. The protein levels of TRAIL and IDO1 were determined by western blotting analysis. Results were representative of three independent experiments ( ∗ P < 0.05, ∗∗ P < 0.01 and ∗∗∗ P < 0.001, n = 3).

Article Snippet: The membranes were blocked with a solution of 0.1% ( v / v ) Tween-20/TBS (TBS/T) containing 5% ( w / v ) nonfat milk powder for 1 hour at room temperature and then incubated with appropriate primary antibodies against TRAIL, IDO1, phosphor-STAT1, phosphor-STAT2, phosphor-JNK, phosphor-MAPK, or beta-actin (TRAIL from RD, IDO1 from CST, and others from Arigo) overnight at 4°C.

Techniques: Expressing, Real-time Polymerase Chain Reaction, Western Blot

Journal: Stem Cells International

Article Title: The Different Effects of IFN- β and IFN- γ on the Tumor-Suppressive Activity of Human Amniotic Fluid-Derived Mesenchymal Stem Cells

doi: 10.1155/2019/4592701

Figure Lengend Snippet: IFN- β interacted with IFN- γ to promote TRAIL expression and counteracted IDO1 expression initiated by IFN- γ in AFMSCs by regulating the phosphorylation of STAT1/2. 1-MT downregulated TRAIL expression in IFN- β - and IFN- γ -primed AFMSCs by inhibiting the phosphorylation of STAT1/2, but upregulated the IDO1 expression in IFN- γ -primed AFMSCs by intensifying the phosphorylation of JNK/MAPK38 (Supplemental ). (a) AFMSCs were primed for 12 hours with the indicated combinations of cytokines (20 ng/ml each) with or without Jak1 inhibitor (15 μ M). The protein levels of TRAIL, IDO1, and phosphorylated STAT1/2 were determined by western blotting analysis. (b) AFMSCs were treated as described in (a), in addition to setting a 1-MT (0.5 mM) combination. Results were representative of three independent experiments.

Article Snippet: The membranes were blocked with a solution of 0.1% ( v / v ) Tween-20/TBS (TBS/T) containing 5% ( w / v ) nonfat milk powder for 1 hour at room temperature and then incubated with appropriate primary antibodies against TRAIL, IDO1, phosphor-STAT1, phosphor-STAT2, phosphor-JNK, phosphor-MAPK, or beta-actin (TRAIL from RD, IDO1 from CST, and others from Arigo) overnight at 4°C.

Techniques: Expressing, Western Blot